Author(s): Cobra Moradian, Mohammad Reza Fazeli, Dariush Abedi
"High expression of therapeutic proteins is crucial for industrial scale manufacturing. In the present study, induction conditions for the production of interferon β-1b by recombinant Escherichia coli BL21 (DE3) were optimized using response surface methodology based on the central composite design (CCD). The induction conditions included isopropyl β -Dthiogalactopyranoside (IPTG) concentration, induction start time (OD600 nm), post-induction time and temperature. The optimal set of culture conditions for high interferon β-1b was determined: 0.7 mM IPTG, an induction start time (OD600 nm) of 0.58, a post-induction time of 5h, and a post-induction temperature of 37°C. A high level of interferon beta-1b protein was expressed in the optimized conditions, which accounted for about 40.2 % of the total cell proteins."
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