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In vitro mutation induction on TCL explants of Lilium (Lilium spp.) with Ethyl Methane Sulfunate (EMS)


Author(s): Fatemeh Keykha Akhar, Azade Khadem, Ahmad Sharifi, Zahra Nemati, Mahbobe Yazdi, Abdolreza Bagheri

"In vitro mutagenesis is considered as an efficient approach for increasing the plant diversity. Lilium is one of the important bulbous flowers that distributed widely over the world. The present study was carried out to investigate the effect of Ethyl Methane Sulfunate (EMS) on in vitro mutagenesis of Lilium spp. (cv. OT Geel and Robina). After surface sterilization bulb scales, they were treated with EMS solution. Then, they were cut into 3mm thickness TCL explants and incubated on regeneration medium. Results indicated that 0.2% EMS was the most effective concentration in enhancement of shoots numbers in both cultivars. Molecular changes in OT Geel and Robina mutants showed that mutation can induce high polymorphism, so that in OT Geel, 11 treatments were classified into 4 groups with similarity level 0.87% and in Robina, 11 treatments were grouped into 2 groups with similarity level 0.86%. In all treatments genetic variations were observed, but higher concentrations of EMS were more effective for mutation induction. The results indicate that the efficiency of ISSR marker for detection of genetic variants is high and leads to early selection of them. This study is the first report to identify EMS treated mutants using ISSR marker in Lilium plants."

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