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Electrochemical Genosensing of Leishmania major using Gold Hierarchical Nanoleaflets

Abstract

Author(s): Marzieh Moradi, Naghmeh Sattarahmady, Gholamreza Hatam, Hossein Heli

"A label- and PCR-free electrochemical genosensor was fabricated for identification of Leishmania parasites. Gold hierarchical nanoleaflets were synthesized by electrodeposition in the presence of spermidine in the deposition solution. A Leishmania major specific single stranded DNA probe was immobilized on the surface of nanoleaflets and ferrocyanide was employed as a hybridization marker. Hybridization of the immobilized probe with targets was investigated under the selected conditions. The genosensor could detect a synthetic DNA target in a range of 1.0×10-12 to 1.0×10-20 mol L-1 with a limit of detection of 2.9810-21 mol L-1, and genomic DNA in a range of 0.5 to 15 ng μL-1 with a limit of detection of 0.11 ng μL-1. The genosensor was successfully applied for detection of Leishmania major in patient samples."

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