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Effects of hydro-alcoholic extract of broccoli (Brassica oleracea) on sensory threshold of pain using the for-malin test in adult male rats


Author(s): Elham Danesh, Saeid Khatamsaz, Manzarbano Shojaeifard, Zahra Khabbaz

"The study of plant species that have been traditionally used as painkillers is a useful and logical research strategy in the search for new anti-nociceptive compounds. The aim of the present study was to evaluate the effect of oral administration of the Brassica oleraceahydroalcoholic extract by formalin test in rats. In this research, 50 male- Wistar rats weighting about 210± 20 grams were divided into 5 groups (n=10). For evaluation of antinociceptive effects, the formalin test induced pain. The nociceptive response develops two phases: First (0-5) min after formalin (first or acute phase) and (16-60) min after formalin injection (second or chronic phase). The animals pre-treated with oral doses of Brassica oleracea extracts (500, 1000, 2000 mg/kg), one month before administration formalin. The control group without receiving any drug and the sham group receiving 1ml hydro alcoholic extract one month before administration formalin. A statistical analysis by ANOVA and T-Test used (p< /05). The data shows there is decreased pain in the formalin test in the group that received 2000 mg/kg dose of extract in comparison with the control and sham groups ( P< /05). It was concluded that probably, vitamin c, sulforaphane and flavonoids are important compound of the extraction decrease pain. Brassica oleraceahydroalcoholic extract by decrease of intracellular ca2+ and ca2+ - dependently enzyme activity inhibition such as phospholipase A2 and iNOS that decreased NO and prostaglandins consequently causes that decrease pain."

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