Thioglycollate broth with Liver Extract (BQ vaccine medium) was used to produce mass scale yield of Clostridium perfringens Type D. Upon growth of Clostridium perfringens in different culture media the OD value of bacterial growth in BQ vaccine medium, Reinforced Culture Medium (RCM), Cooked Meat Medium (CMM) and Nutrient Broth (NB) was 2.260, 2.184, 2.096 and 1.984 respectively. Mice Lethality result of growth in BQ vaccine medium was equal to that of RCM while growth in CMM and NB had very less lethality. ELISA percentage positivity for Epsilon toxins in the supernatant of growth in BQ medium at 450 nm wavelength at dilution of 320 and 640 was higher than that of all the culture media under comparison. It was concluded that BQ was an excellent culture medium for the development of Enterotoxaemia vaccine producing maximum concentration of epsilon toxin than any culture media used before.
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