Journal of Biology and Today's World

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Comparison of Thioglycollate Broth with Liver Extract (BQ Vaccine Medium) with Traditionally Used Culture Media for Mass Scale Production of Clostridium perfringens Type D


Author(s): Azam Ali Nasir, Sohail Manzoor*, Nofil Mustafa, Asma Kausar, Muhammad Usman Ashraf and Muhammad Imran

Thioglycollate broth with Liver Extract (BQ vaccine medium) was used to produce mass scale yield of Clostridium perfringens Type D. Upon growth of Clostridium perfringens in different culture media the OD value of bacterial growth in BQ vaccine medium, Reinforced Culture Medium (RCM), Cooked Meat Medium (CMM) and Nutrient Broth (NB) was 2.260, 2.184, 2.096 and 1.984 respectively. Mice Lethality result of growth in BQ vaccine medium was equal to that of RCM while growth in CMM and NB had very less lethality. ELISA percentage positivity for Epsilon toxins in the supernatant of growth in BQ medium at 450 nm wavelength at dilution of 320 and 640 was higher than that of all the culture media under comparison. It was concluded that BQ was an excellent culture medium for the development of Enterotoxaemia vaccine producing maximum concentration of epsilon toxin than any culture media used before.

Share this article

Reach Us

Avenue Roger Vandendriessche 18,
1150 Brussels, Belgium
Toll Free +32 (800) 709-48

Contact Us

E-Mail: [email protected]

© Lexispublisher 2020 | Creative Commons License Open Access Journals by Lexispublisher is licensed under a Creative Commons Attritbution 4.0 International License